Enhanced upconversion nanoparticles as turn-on fluorescent nanosensors for determination of Porcine epidemic diarrhea virus.
📄 英文摘要 English Abstract
Porcine epidemic diarrhea virus (PEDV) can cause diarrhea, vomiting, dehydration and high mortality in pigs, resulting in significant economic losses for the swine industry. The current research intelligently converges core-shell upconversion nanoparticles (CSUCNPs) and molybdenum disulfide nanosheets modified magnetic nanoparticles (MNPs@MoS2) to achieve the diagnosis of PEDV. The scheme employs the inner filter effect via between CSUCNPs and MNPs@MoS2. With the existence of PEDV, the aptamers bind to PEDV preferentially and interrupt the IFE, thus leading the recoveries of upconversion fluorescence. Therefore, PEDV can be quantified by the linear curve between fluorescence intensity and logarithm concentration of PEDV at the range of 0.01-104 μg/L. This proposed nanosensor exhibits a satisfied detection of limit as 0.021 μg/L. Importantly, a real sample test was performed and compared with standard method, the results suggested the nanosenor demonstrates excellent accuracy and reproducibility, and has promising application prospects.
📄 中文摘要 Chinese Abstract
📋 英文结构化总结 English Structured Summary
摘要整理
Background:
Porcine epidemic diarrhea virus (PEDV) can cause diarrhea, vomiting, dehydration and high mortality in pigs, resulting in significant economic losses for the swine industry.
Methods:
The current research intelligently converges core-shell upconversion nanoparticles (CSUCNPs) and molybdenum disulfide nanosheets modified magnetic nanoparticles (MNPs@MoS2) to achieve the diagnosis of PEDV. The scheme employs the inner filter effect via between CSUCNPs and MNPs@MoS2. With the existence of PEDV, the aptamers bind to PEDV preferentially and interrupt the IFE, thus leading the recoveries of upconversion fluorescence.
Results:
PEDV can be quantified by the linear curve between fluorescence intensity and logarithm concentration of PEDV at the range of 0.01-104 μg/L. This proposed nanosensor exhibits a satisfied detection of limit as 0.021 μg/L. A real sample test was performed and compared with standard method, and the results suggested the nanosensor demonstrates excellent accuracy and reproducibility, and has promising application prospects.
Data Summary:
The quantitative detection range was 0.01-104 μg/L, based on the linear curve between fluorescence intensity and logarithm concentration of PEDV. The satisfied detection of limit was 0.021 μg/L.
Conclusions:
The proposed nanosensor demonstrates excellent accuracy and reproducibility in a real sample test compared with standard method, and has promising application prospects for the diagnosis of PEDV.
Practical Significance:
The nanosensor provides a real sample test approach for the diagnosis of PEDV, with promising application prospects in the swine industry.
📋 中文结构化总结 Chinese Structured Summary
背景:
猪流行性腹泻病毒（PEDV）可引起猪腹泻、呕吐、脱水和高死亡率，给养猪业造成重大经济损失。
方法:
本研究巧妙地将核壳结构上转换纳米粒子（CSUCNPs）与二硫化钼纳米片修饰的磁性纳米粒子（MNPs@MoS2）相结合，以实现PEDV的诊断。该方案利用CSUCNPs与MNPs@MoS2之间的内滤效应。当存在PEDV时，适配体优先与PEDV结合并阻断内滤效应，从而使上转换荧光恢复。
结果:
在0.01-104 μg/L范围内，PEDV可通过荧光强度与PEDV对数浓度之间的线性曲线进行定量检测。所提出的纳米传感器表现出令人满意的检测限，为0.021 μg/L。进行了实际样品检测并与标准方法进行比较，结果表明该纳米传感器具有优异的准确度和重复性，且具有广阔的应用前景。
数据摘要:
定量检测范围为0.01-104 μg/L，基于荧光强度与PEDV对数浓度之间的线性曲线。令人满意的检测限为0.021 μg/L。
结论:
与标准方法相比，所提出的纳米传感器在实际样品检测中表现出优异的准确度和重复性，并在PEDV诊断中具有广阔的应用前景。
实际意义:
该纳米传感器为PEDV诊断提供了一种实际样品检测方法，在养猪业中具有广阔的应用前景。