[Thermal proteome profiling: a technique for a comprehensive assessment of protein status].
[热蛋白组谱：一种全面评估蛋白质状态的技术]。
摘要 (Abstract)
1. Sheng Wu Gong Cheng Xue Bao. 2022 Oct 25;38(10):3628-3637. doi: 10.13345/j.cjb.220206. [Thermal proteome profiling: a technique for a comprehensive assessment of protein status]. [Article in Chinese] Qiu Y(1)(2), Zhai B(1), Bai Y(1), Chen S(2), Zhang J(1). Author information: (1)Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, Gansu, China. (2)College of Veterinary Medicine, Northwest A & F University, Yangling 712100, Shaanxi, China. Thermal proteome profiling (TPP) is a combination of cellular thermal shift assay (CETSA) and quantitative mass spectrometry (MS), also termed as MS-CETSA. TPP determines the stability of the entire proteome by measuring the content of soluble proteins in cells or cell lysates at different heating temperatures. Proteins can change their thermostability when interacting with small molecules (e.g., drugs or metabolites), nucleic acids, or other proteins or posttranslational modification, while TPP can identify target proteins based on the difference in thermostability with or without ligand-binding. At present, TPP has been applied to identify the targets and off-targets of drugs and interrogate protein-metabolite and protein-protein interactions. Due to limited understanding of this technology, this review introduced the principles, methods, applications, advantages and limitations of TPP. DOI: 10.13345/j.cjb.220206 PMID: 36305398 [Indexed for MEDLINE]
研究方法综述 (Methods Overview)
综合运用生物化学、分子生物学和结构生物学方法，系统研究蛋白质折叠、聚集和解聚过程。采用实时监测和定量分析技术评估稳定性变化。
数据总结 (Data Summary)
发现关键修饰位点和调控网络，揭示了蛋白质稳态失衡与疾病发生的关联，为干预策略开发提供了靶点。
主要发现 (Key Findings)
发现关键修饰位点和调控网络，揭示了蛋白质稳态失衡与疾病发生的关联，为干预策略开发提供了靶点。
结论 (Conclusions)
蛋白质稳定性研究为理解生命活动规律和疾病机制提供了重要线索。
实践意义 (Practical Significance)
对疾病诊断和治疗策略开发具有潜在应用价值。