Metabolic Engineering of Microbial Cell Factories for Efficient Heme Biosynthesis: Advances, Challenges, and Future Perspectives
微生物细胞工厂高效合成血红素的代谢工程：进展、挑战与未来展望
📄 英文摘要 English Abstract
, for efficient heme biosynthesis. While biosynthetic titers have improved significantly through modular optimization and excretion engineering, heme's stability and functional mechanisms under industrial food processing conditions remain unsystematically reviewed. In this work, we also emphasize the thermomechanical fate and catalytic role of microbial heme during food processing. We analyze how biochemical pathway diversity (PPD, CPD, and SHD routes) informs host-specific engineering strategies while examining heme's stability and its role in oxidative cross-linking of proteins under extreme heat and high shear. This review provides a comprehensive framework for engineering next-generation, high-performance sustainable foods.
📄 中文摘要 Chinese Abstract
📋 英文结构化总结 English Structured Summary
摘要整理
Background:
While biosynthetic titers have improved significantly through modular optimization and excretion engineering, heme's stability and functional mechanisms under industrial food processing conditions remain unsystematically reviewed. In this work, we also emphasize the thermomechanical fate and catalytic role of microbial heme during food processing.
Methods:
N/A - Review article. We analyze how biochemical pathway diversity (PPD, CPD, and SHD routes) informs host-specific engineering strategies while examining heme's stability and its role in oxidative cross-linking of proteins under extreme heat and high shear.
Results:
We analyze how biochemical pathway diversity (PPD, CPD, and SHD routes) informs host-specific engineering strategies while examining heme's stability and its role in oxidative cross-linking of proteins under extreme heat and high shear.
Data Summary:
No quantitative results or key statistics are provided in the abstract.
Conclusions:
This review provides a comprehensive framework for engineering next-generation, high-performance sustainable foods.
Practical Significance:
This review provides a comprehensive framework for engineering next-generation, high-performance sustainable foods.
📋 中文结构化总结 Chinese Structured Summary
背景:
尽管通过模块化优化和排泄工程，生物合成滴度已显著提高，但血红素在工业食品加工条件下的稳定性和功能机制仍未得到系统综述。在本工作中，我们还强调了微生物血红素在食品加工过程中的热力学命运及其催化作用。
方法:
不适用——综述文章。我们分析了生化途径多样性（PPD、CPD和SHD途径）如何指导宿主特异性工程策略，同时考察了血红素的稳定性及其在极端高温和高剪切条件下对蛋白质氧化交联的作用。
结果:
我们分析了生化途径多样性（PPD、CPD和SHD途径）如何指导宿主特异性工程策略，同时考察了血红素的稳定性及其在极端高温和高剪切条件下对蛋白质氧化交联的作用。
数据摘要:
摘要中未提供定量结果或关键统计数据。
结论:
本综述为开发下一代高性能可持续食品的工程化提供了全面的框架。
实际意义:
本综述为开发下一代高性能可持续食品的工程化提供了全面的框架。