REVIEW published: 24 June 2021 doi: 10.3389/fcell.2021.686173
Involvement of TRP Channels in Adipocyte Thermogenesis: An Update Wuping Sun 1† , Yixuan Luo 2† , Fei Zhang 2 , Shuo Tang 3* and Tao Zhu 4* 1
Department of Pain Medicine and Shenzhen Municipal Key Laboratory for Pain Medicine, Shenzhen Nanshan People’s Hospital and The 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China, 2 Department of Cardiovascular Surgery, Shenzhen Nanshan People’s Hospital and The 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China, 3 Department of Orthopaedics, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, China, 4 Department of Respiratory Medicine, Second Affiliated Hospital of Chongqing Medical University, Chongqing, China
Edited by: Maria Elena De Lima, Grupo Santa Casa BH, Brazil Reviewed by: Karla S. Fernandes, Instituto de Ensino e Pesquisa Santa Casa BH, Brazil Célio José Castro Junior, Grupo Santa Casa BH, Brazil *Correspondence: Tao Zhu zhutao063020@163.com Shuo Tang tangshuo1205@163.com
Obesity prevalence became a severe global health problem and it is caused by an imbalance between energy intake and expenditure. Brown adipose tissue (BAT) is a major site of mammalian non-shivering thermogenesis or energy dissipation. Thus, modulation of BAT thermogenesis might be a promising application for body weight control and obesity prevention. TRP channels are non-selective calcium-permeable cation channels mainly located on the plasma membrane. As a research focus, TRP channels have been reported to be involved in the thermogenesis of adipose tissue, energy metabolism and body weight regulation. In this review, we will summarize and update the recent progress of the pathological/physiological involvement of TRP channels in adipocyte thermogenesis. Moreover, we will discuss the potential of TRP channels as future therapeutic targets for preventing and combating human obesity and related-metabolic disorders. Keywords: TRP channels, calcium, thermogenesis, energy metabolism, brown adipocytes, beige adipocytes, obesity
equally to this work Specialty section: This article was submitted to Cellular Biochemistry, a section of the journal Frontiers in Cell and Developmental Biology Received: 26 March 2021 Accepted: 02 June 2021 Published: 24 June 2021 Citation: Sun W, Luo Y, Zhang F, Tang S and Zhu T (2021) Involvement of TRP Channels in Adipocyte Thermogenesis: An Update. Front. Cell Dev. Biol. 9:686173. doi: 10.3389/fcell.2021.686173
ADIPOSE TISSUES AND OBESITY Obesity is a severe public health problem causing various diseases including diabetes, hypertension, coronary heart diseases and cancer, which has received considerable attention as a major public health concern (Nguyen and El-Serag, 2010; Blüher, 2019). According to a prediction based on the data from 1975 to 2014 in 200 countries, the prevalence of global obesity will reach to 18% for men and 21% for women by 2025 (NCD Risk Factor Collaboration, 2016). In addition, obesity is becoming prevalent not only in the developed countries, but also in the developing countries (Maharani and Tampubolon, 2016). Therefore, urgent strategies are required for the prevention and reversal of obesity and related metabolic diseases. Abbreviations: BMI, body mass index; BAT, brown adipose tissue; CT, computed tomography; FDG, fluorodeoxyglucose; HFD, high fat diet; iBAT, interscapular BAT; WAT, white adipose tissue; UCP1, uncoupling protein-1; PET, positron emission tomography; PGC1α, peroxisome proliferator-activated receptor gamma coactivator 1-alpha; ROS, reactive oxygen species; TM, trans-membrane; TRP channel, Transient receptor potential channel; TRPV, TRP Vanilloid; TRPV2KO, TRPV2knockout; TRPC, TRP Canonical; TRPM, TRP Melastatin; TRPML, TRP Mucolipin; TRPN, TRP NomPC; TRPP, TRP Polycystin; TRPA, TRP Ankyrin; WT, wild-type; [Ca2+ ]i , intracellular Ca2+ levels; 4α-PDD, 4α-phorbol-12, 13-didecanoate.
Frontiers in Cell and Developmental Biology | www.frontiersin.org 1 June 2021 | Volume 9 | Article 686173 Sun et al. TRP Channels in Adipocyte Thermogenesis
Obesity is accompanied by the imbalance of caloric intake and consumption (Hall and Guo, 2017). There is evidence that adipose tissue is involved in the long-term regulation of energy metabolism and fat quality. Adipose tissue is a highly specialized tissue and plays a key role in energy mobilization regulation (Reilly and Saltiel, 2017; Zhai et al., 2020). Two types of adipose tissue have been found in mammals so far, called white adipose tissue (WAT) and brown adipose tissue (BAT) (Cannon and Nedergaard, 2004; Wu et al., 2020). WAT is generally thought as an organ stores excess energy which maintains energy in the form of triglyceride in lipid droplets. However, a new type of brown-like adipocyte was termed beige/brite adipocyte or inducible brown adipocyte has recently been found in human WAT (Sharp et al., 2012; Cypess et al., 2013; Lidell et al., 2013). BAT, which consumes energy and produce heat rapidly, was first discovered in mammalian hibernation research (Ricquier and Kader, 1976). This thermogenic function is mainly mediated by uncoupling protein-1 (UCP1), a polypeptide that exists in the mitochondrial inner membrane of brown adipocytes (Kajimura et al., 2015; Bertholet et al., 2017; Cannon et al., 2020). It has assessed that BAT thermogenesis was decreased in obese mice by oxygen consumption measurement (Martinez-Botas et al., 2000; Ussher et al., 2010). UCP1 expression level in BAT was decreased in almost all obese animals whereas increased in lean animals (Shirkhani et al., 2018). UCP1 knockout (UCP1KO) mice exhibited obesity phenotypes with increased body fat after six months high fat diet (HFD) feeding (Kontani et al., 2005). On the other hand, cold stimulation and/or β3-adrenergic receptor agonist treatment decreased body fat amount by enhancing BAT activity (Lowell and Spiegelman, 2000; Cannon and Nedergaard, 2004). Cold exposure also increased BAT volume and activity, thus increasing energy consumption and promoting weight loss of obese people (Hanssen et al., 2015a,b; Leiria et al., 2019). Several studies have reported that there was a negative correlation between BAT activity/amount and body mass index (BMI) in humans. Imaging data have revealed that patients with higher BMI have lower BAT activity (Cypess et al., 2009; Pfannenberg et al., 2010; Ouellet et al., 2011). Moreover, a single nucleotide substitution at –3826A to G of UCP1 gene polymorphism has been found in human, which decreased the mRNA expression of Ucp1 and enhanced the age-related obesity and BAT degradation (Nagai et al., 2007; Yoneshiro et al., 2013). Therefore, BAT might play critical role in the regulation of body weight and energy homeostasis.
Classical brown fat is primarily distributed around interscapular BAT (iBAT), axillary, paravertebral, and perirenal sites (Park et al., 2014). Mitochondria and multilocular lipid droplets were enriched in brown adipocytes, which makes it have remarkable capacity to dissipate energy in the form of heat (Song et al., 2020). UCP1 is expressed in the mitochondria inner membranes of brown adipocytes, which uncouples ATP synthesis from oxidative phosphorylation, thereby dissipating energy as heat. It is well known that BAT non-shivering thermogenesis is controlled directly by sympathetic nervous system innervation and activation. BAT thermogenesis is induced and regulated by the release of norepinephrine from sympathetic nerve terminals and its subsequent binding by β3-adrenergic receptors (Nedergaard et al., 2005; Feldmann et al., 2009). Several studies have shown that how UCP1 is activated, and long chain fatty acid is essential for H+ transport (Fedorenko et al., 2012). In addition, another proposed mechanism is that mitochondrial reactive oxygen species (ROS) production regulates UCP1 sulfenylation and thermogenesis (Chouchani et al., 2016). However, signaling pathways for thermogenesis in the downstream of β3-adrenergic receptor activation still have not been well clarified. Beige adipocyte (UCP1-positive adipocyte) is known to be surrounded by numerous UCP1-negative adipocytes in human WAT (Wu et al., 2012). Beige adipocytes could be recruited after a short-term cold challenge or treatment with β3-adrenergic receptor agonists (Saito et al., 2020). They are very similar to brown adipocytes with high UCP1 expression and thermogenesis (Ye et al., 2013; Li et al., 2014). There are two groups that are a BAT-positive group (subjects have detectable FDG uptake upon cold stimulation) and a BAT-negative group (subjects have undetectable FDG uptake) in humans. Energy metabolism was higher in the BAT-positive group than the BAT-negative group after an acute cold exposure (Orava et al., 2011; Yoneshiro et al., 2011). These studies clearly revealed a critical function for brown and beige adipocytes in cold-induced thermogenesis in humans. Therefore, approaches to modulate brown or beige adipocyte activities might be potential way to prevent and treat human obesity and related metabolic diseases.
TRP CHANNELS Transient receptor potential (TRP) ion channels are a major class of calcium-permeable channels, most of which are non-selective cation channels (Montell and Rubin, 1989). TRP channels contain six trans-membrane (TM) domains (TM1–TM6) with a pore loop between TM5 and TM6 (Cao et al., 2013b; Liao et al., 2013; Paulsen et al., 2015; Huynh et al., 2016; Zubcevic et al., 2016). TRP channel superfamily is now subdivided into seven subfamilies and contains 27 channels: TRPV (Vanilloid), TRPC (Canonical), TRPM (Melastatin), TRPML (Mucolipin), TRPN (NomPC), TRPP (Polycystin), and TRPA (Ankyrin) based on their primary amino acid sequences (Ramsey et al., 2006; Wu et al., 2010; Gees et al., 2012). The main signaling pathways in which TRP channels triggered are based on calcium influx through the channels, leading to increases in intracellular Ca2+ levels ([Ca2+ ]i ). Numerous studies have shown that some TRP channels are expressed in adipocytes and are involved in energy
THERMOGENESIS IN BROWN AND BEIGE ADIPOCYTES BAT was thought to be restricted only in infants (Lean, 1989; Enerback, 2010). However, previous works have reported that BAT was also found in adult humans by using fluorodeoxyglucose (FDG)-positron emission tomography (PET) in combination with computed tomography (CT) techniques (Cypess et al., 2009; van Marken Lichtenbelt et al., 2009). This novel finding highlights the critical role for BAT in the regulation of energy metabolism and fat deposition (Nedergaard and Cannon, 2010; Nedergaard et al., 2011). Frontiers in Cell and Developmental Biology | www.frontiersin.org
prevalence of human obesity in eastern Asian countries was decreased by increasing the consumption of hot foods containing capsaicin (Wahlqvist and Wattanapenpaiboon, 2001). It has also been reported that capsaicin injection induced adrenaline secretion, this effect was significantly reduced in TRPV1KO mice (Uchida et al., 2017). Capsaicin directly binds to TRPV1 in gastrointestinal tract, produce afferent signal, subsequently transmit to ventromedial hypothalamic nucleus of central nervous system, and finally send signal to WAT. This could promote the expression of β2-adrenoceptor and the production of PRDM16 protein, thus promoting the generation of beige adipocytes, resulting in increased systemic energy expenditure (Ohyama et al., 2016; Saito et al., 2020). Catechins in green tea may activate and recruit BAT by acting on TRPV1/TRPA1 of gastrointestinal sensory neurons in the same way as capsaicin (Mako et al., 2015). Besides, topical application of capsaicin cream in mice resulted in weight loss and adipose tissue weight (Lee et al., 2013). However, whether the regulatory effect of topical capsaicin on obesity is through TRPV1 to activate central nervous system remains to be further studied. These studies clearly demonstrated that targeting TRPV1 and modulation its activity with capsaicin and analogs could be effective approaches for human obesity treatment and prevention, although the anti-obesity effect of TRPV1 activation may be involved not only in adipose tissue, but also in nervous system.
metabolism and inflammation of adipose tissues, suggesting the potential role of TRP channels in human obesity treatment and prevention (Bishnoi et al., 2018; Uchida et al., 2018; Gao et al., 2019; Zhai et al., 2020). In the present review, we will provide a systematic and brief summary of TRP channels in the regulation of adipocyte thermogenesis and update the recent progress.
TRPV1 TRPV1 is well-known as a receptor of capsaicin, the pungent ingredient in “hot” chili peppers (Caterina et al., 1997). TRPV1 is activated by a variety of stimuli, including heat (Cao et al., 2013a), protons and capsaicin (Dhaka et al., 2009). In addition, TRPV1 is activated by some compounds in garlic, onion (Salazar et al., 2008), black pepper (Okumura et al., 2010), and other foods, such as gingerol (Iwasaki et al., 2006). TRPV1 has been reported to be expressed in both WAT and BAT (Bishnoi et al., 2013; Kida et al., 2016). TRPV1 expression level is increased in the differentiated HB2 brown adipocytes than in pre-adipocytes (Kida et al., 2016). Moreover, activation of TRPV1 up-regulates the expression of thermogenic genes and induced “browning” in 3T3-L1 adipocytes (Figure 1; Baboota et al., 2014). TRPV1 is expressed in 3T3-L1 pre-adipocytes, adipose tissue of mice and fat tissue of obese humans (Zhang et al., 2007). TRPV1 is activated by dietary capsaicin, a process that induces calcium influx and prevents adipogenesis in 3T3-L1 cells (Zhang et al., 2007) and probably occurs through a calcineurin pathway (Cioffi, 2007). Besides, dietary capsaicin treatment prevented HFDinduced obesity in wild-type (WT) mice in vivo, but not in TRPV1KO mice (Zhang et al., 2007; Chen J. et al., 2015; Chen N. et al., 2015). Moreover, TRPV1 was involved in the regulation of energy intake and glucose homeostasis in WAT during HFDinduced obesity (Lee et al., 2015). Absence of TRPV1 exacerbated obese and insulin resistance associated with HFD and aging (Lee et al., 2015). It has also been reported that monoacylglycerol up-regulated UCP1 expression level in brown adipocytes and suppressed accumulation of visceral fat in mice fed with high fat and sucrose through activation of TRPV1 (Iwasaki et al., 2011). Fish oil intake induced UCP1 up-regulation in both brown and white adipose tissues in a TRPV1 dependent manner (Kim et al., 2015; Lund et al., 2018). Oleoylethanolamide, a newly reported TRPV1 ligand, is also involved in the regulation of energy intake and consumption, feeding behavior and weight control (Laleh et al., 2019). Human studies have showed that capsaicin ingestion enhanced fat oxidation and energy metabolism during aerobic exercise (Shin and Moritani, 2007). A continuous consumption of chili increased the energy metabolism in the middle-aged subjects (Ahuja et al., 2006). Capsinoids, a non-pungent capsaicin analogs, for continuous 1–3 months also increased energy expenditure and fat oxidation with a reduction in abdominal adiposity in overweight and obese subjects (Inoue et al., 2007; Snitker et al., 2009). Moreover, capsaicin and capsinoids as food ingredients enhanced BAT thermogenesis, subsequently decreased fat mass in humans (Yoneshiro et al., 2012; Saito and Yoneshiro, 2013). An epidemiological study suggested that the energy metabolism was enhanced and the Frontiers in Cell and Developmental Biology | www.frontiersin.org
TRPV2 TRPV2 was initially reported to be activated by noxious heat with an activation temperature threshold of higher than 52◦ C (Caterina et al., 1999) and found to be activated by several chemicals, e.g., 2-aminoethoxydiphenyl borate (2APB) and lysophosphatidylcholine (LPC) (Juvin et al., 2007; Monet et al., 2009). TRPV2 was also reported to be activated by mechanical stimulation and/or cell swelling (Muraki et al., 2003; Iwata et al., 2009). TRPV2 is expressed in both WAT and BAT (Sun et al., 2017a). TRPV2 is highly expressed in mouse brown adipocytes compared with TRPV1, TRPV3, TRPV4 and TRPM8 (Sun et al., 2016a,b). The expression of TRPV2 was up-regulated at mRNA, protein and functional levels in the differentiated brown adipocytes (Sun et al., 2016b, 2017b). Primary TRPV2-deficient (TRPV2KO) adipocytes show decreased mRNA levels of multiple genes involved in mitochondrial oxidative metabolism, such as Ucp1 and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (Pgc1α). Besides, TRPV2KO adipocytes showed decreased responses to a β-adrenergic receptor agonist, isoproterenol, which might be due to the lack of TRPV2mediated calcium influx. These results suggested that TRPV2mediated calcium influx is involved in thermogenic gene induction upon β-adrenergic receptor activation (Figure 1). TRPV2KO mice showed cold intolerance and significantly smaller increases in Ucp1 mRNA and protein upon cold stimulation at 4◦ C without changes in their activities. On the other hand, sympathetic nerve activity was not changed in TRPV2KO mice. TRPV2KO mice showed impaired iBAT adaptive thermogenesis upon administration of a β3-adrenergic 3
FIGURE 1 | TRP channel-mediated adipocyte thermogenesis. A schematic figure of how TRPV1, TRPV2, TRPV4, TRPM8, and TRPC1-mediated calcium influx regulates thermogenic gene expression in adipocytes, which causes enhanced thermogenesis. Moreover, the increase in sympathetic nerve activity causes norepinephrine release from the sympathetic nerves and activation of β3-adrenergic receptor (β3ADR) in brown adipocytes, TRPV2 synergistically collaborated with β3ADR to involve in the regulation of peroxisome proliferator-activated receptor gamma coactivator-1 a (PGC1a) and uncoupling protein 1 (UCP1), subsequently enhances thermogenesis. On the other hand, TRPV4-mediated calcium influx negatively regulates thermogenic gene expression in adipocytes and subsequently inhibits thermogenesis.
an anti-adipogenic role in vivo (Cheung et al., 2015). Besides, berberine alleviates olanzapine-induced obesity by targeting TRPV1/TRPV3 in hypothalamus of mice (Singh et al., 2020). These studies suggested that targeting TRPV3 could be an intriguing approach for the treatment and prevention of obesity. However, the expression of TRPV3 and its role in human obesity needs further exploration.
receptor agonist, BRL37344. Importantly, TRPV2KO mice had significant increases in body weight and adipose tissues upon a HFD treatment (Sun et al., 2016a). Up-regulation of TRPV2 was also observed in obese and diabetic (db/db) mice (Sun et al., 2016a, 2017a). It has also been reported that tart cherry may attenuate adipogenesis by acting directly on the adipose tissue and down-regulating the HFD-induced mRNA expression of TRPV1 and TRPV2 (Cocci et al., 2021). These findings suggested that TRPV2 might be contributed to adipocyte thermogenesis. However, it is necessary to further examine the expression and function of TRPV2 in human BAT and develop specific ligands of TRPV2.
TRPV4 TRPV4 was reported to be activated by osmolarity changes or mechanical stimuli (Liedtke et al., 2000; Strotmann et al., 2000; Watanabe et al., 2002a). TRPV4 is also activated by diverse chemical compounds, including a synthetic phorbol ester, 4α-phorbol-12, 13-didecanoate (4α-PDD) and GSK1016790A (Watanabe et al., 2002b; Willette et al., 2008) as well as moderate warmth (temperature threshold higher than 27◦ C) (Guler et al., 2002; Watanabe et al., 2002b). TRPV4 is expressed in BAT and WAT as well (Sun et al., 2017a, 2020; Uchida et al., 2018). It has been reported that insulin regulates TRPV4-mediated metabolic homeostasis in human white adipocytes (Sanchez et al., 2016). TRPV4 is involved in the modulation of thermogenic and inflammatory pathways in adipose tissue. Knockdown of TRPV4 enhanced the basal and norepinephrine-induced induction of the expression of Pgc1a and Ucp1 (Ye et al., 2012). ERK1/2 were reported to be activated by TRPV4-mediated calcium signaling (Thodeti et al., 2009), and TRPV4 activation-induced calcium influx caused a rapid phosphorylation of ERK1/2 and JNK1/2, which further suppressed the expression of thermogenic genes in 3T3-F442A adipocytes (Figure 1; Ye et al., 2012). Knockdown of TRPV4 also reduced adipose tissue inflammation by inhibiting a number of pro-inflammatory genes (Ye et al., 2012). The expression of TRPV4 in WAT was higher than that in BAT (Sun et al., 2017a). The significant up-regulation of thermogenic gene expression upon TRPV4 inhibition led to the
TRPV3 TRPV3 is a member of the TRPV subfamily which is different from TRPV1 and TRPV2. TRPV3 is well-known to be activated by innocuous temperature around body temperature but initially activated by a high noxious threshold which is over 50◦ C (Liu and Qin, 2017). The chemical agonists of TRPV3 include camphor, carvacrol, (-)-epicatechin, 2APB, and endogenous ligand farnesyl pyrophosphate (Cheung et al., 2015; Broad et al., 2016). TRPV3 could form heteromeric channels with TRPV1 (Cheng et al., 2012), which also involves in the regulation of adipogenesis and HFD-induced obesity (Cheung et al., 2015). TRPV3 has been reported to be expresses in BAT and WAT (Bishnoi et al., 2013). The expression of TRPV3 was dramatically down-regulated in visceral adipose tissue of obesity mice, including HFD-induced obesity mice, ob/ob and db/db mice (Cheung et al., 2015; Sun et al., 2017a). HFD feeding up-regulated TRPV3 in the medial nucleus tractus solitaries and hypoglossal nucleus, which is accompanied by a reduced expression of proopiomelanocortin and resulted in increased food intake and a gain of bodyweight (Hu et al., 2011). Activation of TRPV3 by (-)-epicatechin prevented adipogenesis in 3T3-L1 preadipocytes and played Frontiers in Cell and Developmental Biology | www.frontiersin.org
by its ligands, such as menthol and icilin, mimics adipocyte thermogenesis and might constitute a promising approach to prevent overweight and obesity. However, randomized clinical trials of topical menthol in obese patients are necessary.
occurrence of WAT “browning” (Ye et al., 2012). TRPV4KO mice exhibited increased muscle energy oxidation and resistance to HFD-induced obese in mice (Kusudo et al., 2012). It has also been reported that treadmill running and rutin ameliorate HFDinduced obesity in mice by suppressing the expression of TRPV4 in adipocytes (Chen N. et al., 2015). Besides, dietary intervention in obese dams protects male offspring from WAT induction of TRPV4, adiposity, and hyperinsulinemia (Janoschek et al., 2016). A human subject-based study has revealed that polymorphisms of TRPV4 gene affects BMI and body fat mass in subjects in Taiwan (Duan et al., 2015). These results revealed an opposite role of TRPV4 in the modulation of adipocyte thermogenesis without knowing the potential mechanisms. Examine the expression and function of TRPV4 in human obesity and developing TRPV4 specific antagonist and in vivo examination of the new compounds is warranted.
TRPA1 TRPA1 was initially reported as a noxious cold-activated channel with a temperature threshold around 17◦ C (Story et al., 2003), However, later studies have initiated a heated debate over the role of TRPA1 as a cold sensor. But its cold sensitivity has been disputed later, and the contribution of TRPA1 to cold sensing is currently a matter of strong debate (Bautista et al., 2006; Talavera et al., 2020). TRPA1 is potentially activated by several food components, like allyl isothiocyanate, icilin, menthol, cinnamaldehyde and capsinoids (Laursen et al., 2015). TRPA1 is involved in adipocyte thermogenesis and energy metabolism (Watanabe and Terada, 2015). In HFD-induced obesity mice, oral administration of allyl isothiocyanate reduces body weight, accumulation of lipid droplets in the liver, and white adipocyte size (Lo et al., 2018). It has been reported that cinnamaldehyde reduces visceral fat deposition in HFDtreated mice by stimulating BAT between scapulae (Tamura et al., 2012). Cinnamaldehyde activates TRPA1 in mouse gastric epithelial cells and up-regulates fatty acid oxidation-related genes in adipose tissue (Camacho et al., 2015). Oleuropein aglycone, as an agonist of TRPA1 and TRPV1, enhances the expression of UCP1 in BAT and promote fat thermogenesis by promoting the secretion of norepinephrine (Oi-Kano et al., 2016). It has been hypothesized that menthol-induced thermogenesis in adipocyte probably involved a TRPA1 mechanism as well (Sakellariou et al., 2016). Moreover, TRPA1 activation induces adrenaline secretion and prevent fat accumulation and obese in rodents (Watanabe and Terada, 2015). Intravenous injection of AITC induces adrenaline secretion, and adrenaline promotes the thermogenesis of BAT by activating β3-adrenergic receptor (Saito et al., 2020). These studies suggested that TRPA1 regulates heat production of BAT through central nervous system (Zsombok and Derbenev, 2016). Therefore, activation of TRPA1 by its ligands might be a promising approach for human obesity treatment and prevention. However, the anti-obesity mechanism which TRPA1 and its ligands involved need further exploration. Randomized clinical trials of TRPA1 activation in obese patients are warranted as well.
TRPM8 The TRPM subfamily consists of eight different subunits, TRPM1 to TRPM8 (Boesmans et al., 2011). TRPM8 is wellknown as a menthol receptor which has been reported in the year of 2002 (McKemy et al., 2002). In a human adipocyte cell line, menthol-induced TRPM8 activation increased UCP1 expression, mitochondrial activation and heat production (Figure 1; Rossato et al., 2014). The mRNA and protein expression levels of TRPM8 are significantly increased in the differentiated adipocytes, suggesting the importance of TRPM8 for adipocyte thermogenesis (Rossato et al., 2014). In cultured adipocytes, menthol induced an up-regulation of UCP1 expression which may through a protein kinase A pathway, which subsequently increases BAT thermogenesis and WAT “browning” (Ma et al., 2012; Jiang et al., 2017; Sanders et al., 2020). Besides, it has been reported that cold-sensing TRPM8 channel participates in the regulation of clock and clock-controlled genes in BAT thermogenesis (Moraes et al., 2017). Bioavailable menthol induces energy expending phenotype in differentiating adipocytes (Khare et al., 2019). In vivo studies have revealed that dietary menthol supplementation dramatically increased the core body temperatures and locomotor activity in WT mice, but not in TRPM8KO and UCP1KO mice. Menthol supplementation in diet alleviated HFD-induced obesity and insulin resistance as well (Ma et al., 2012; Jiang et al., 2017). And the preventive effect of menthol against HFD-induced obesity and related complications probably involve a glucagon mechanism (Khare et al., 2018). These results suggested that activation of TRPM8 could enhance BAT thermogenesis, which paves a new approach for the treatment and prevention of obesity. TRPM8-dependent increase in core body temperature upon a menthol treatment or cold exposure, which may be mediated by a UCP1 up-regulation (Tajino et al., 2011). Intragastric administration of menthol also enhanced BAT thermogenesis in vivo (Tajino et al., 2007; Masamoto et al., 2009). In addition, TRPM8 polymorphism has been reported to be closely correlated with metabolic syndrome in Turkish population (Tabur et al., 2015). Topical menthol appears to increase core body temperature and metabolic rate in adults (Valente et al., 2015). In summary, activation of TRPM8 Frontiers in Cell and Developmental Biology | www.frontiersin.org
TRPC1 AND TRPC5 TRPC subfamily includes seven members (TRPC1–7). TRPC channels are usually formed by homo- or heteromeric TRPC proteins (Huang et al., 2011). There is no evidence demonstrate TRPC channels have thermosensitive property so far. TRPC1 is highly expressed in adipocyte depots including BAT and that TRPC1-deficient mice are prone to weight gain and manifest reduced metabolic control (Wolfrum et al., 2018). TRPC1 regulates brown adipocyte activity in a PPARγ-dependent manner, suggesting that TRPC1 is a downstream component 5
demonstrated to play critical roles in the regulation of energy metabolism for the treatment and prevention of human obesity. In the present review, we summarized and updated the recent progress of the involvement of several TRP channels in adipocyte thermogenesis. It’s worth noting that several concerns still need to be further explored. First of all, the underlying mechanisms which TRP channel-mediated in the thermogenesis process of adipocytes are still controversial, which need to be clearly addressed. Secondly, novel specific ligands of TRP channels are warranted to be developed since there is no specific ligands for TRP channels so far. Thirdly, how do TRP channels exert tissuespecific effects in adipose tissues? These issues are warranted to be addressed by further animal and clinical studies in the future. In conclusion, targeting TRP channels could be promising strategies for clinical treatment and prevention of human obesity and related-metabolic diseases.
of a mechanism that translates metabolic or environmental stimuli into output in the form of BAT activity (Figure 1; Wolfrum et al., 2018). However, an opposite observation has been reported that fat mass and fasting glucose concentrations were lower in TRPC1KO mice that were fed a HFD (45% fat) (Krout et al., 2017). Besides, a mechanically activated TRPC1like current in white adipocytes was observed (El Hachmane and Olofsson, 2018). It has been reported that either knockdown of TRPC1/TRPC5 in vitro or conditional knockout of TRPC5 in vivo has increased adiponectin generation in mouse (Sukumar et al., 2012). In addition, both exogenous and endogenous pituitary adenylate cyclase activating polypeptides stimulate proopiomelanocortin neurons and increase energy consumption by activating TRPC1 and TRPC5 channels, which suggests that it is possible to promote BAT thermogenesis by activating TRPC1/TRPC5 in central nervous system (Chang et al., 2020). These studies demonstrated the involvement of TRPC1/TRPC5 in the regulation of energy homeostasis. Further examination of the expression of TRPC1/TRPC5 in human adipose tissues and developing TRPC1/TRPC5 specific agonist are needed.