[Thermal proteome profiling: a technique for a comprehensive assessment of protein status].
热蛋白质组分析：一种全面评估蛋白质状态的技术
📄 英文摘要 English Abstract
Thermal proteome profiling (TPP) is a combination of cellular thermal shift assay (CETSA) and quantitative mass spectrometry (MS), also termed as MS-CETSA. TPP determines the stability of the entire proteome by measuring the content of soluble proteins in cells or cell lysates at different heating temperatures. Proteins can change their thermostability when interacting with small molecules (e.g., drugs or metabolites), nucleic acids, or other proteins or posttranslational modification, while TPP can identify target proteins based on the difference in thermostability with or without ligand-binding. At present, TPP has been applied to identify the targets and off-targets of drugs and interrogate protein-metabolite and protein-protein interactions. Due to limited understanding of this technology, this review introduced the principles, methods, applications, advantages and limitations of TPP.
📄 中文摘要 Chinese Abstract
📋 英文结构化总结 English Structured Summary
摘要整理
Background:
Thermal proteome profiling (TPP) is a combination of cellular thermal shift assay (CETSA) and quantitative mass spectrometry (MS), also termed as MS-CETSA. TPP determines the stability of the entire proteome by measuring the content of soluble proteins in cells or cell lysates at different heating temperatures. Proteins can change their thermostability when interacting with small molecules (e.g., drugs or metabolites), nucleic acids, or other proteins or posttranslational modification, while TPP can identify target proteins based on the difference in thermostability with or without ligand-binding.
Methods:
N/A - Review article
Results:
While TPP can identify target proteins based on the difference in thermostability with or without ligand-binding. At present, TPP has been applied to identify the targets and off-targets of drugs and interrogate protein-metabolite and protein-protein interactions.
Data Summary:
TPP determines the stability of the entire proteome by measuring the content of soluble proteins in cells or cell lysates at different heating temperatures.
Conclusions:
Due to limited understanding of this technology, this review introduced the principles, methods, applications, advantages and limitations of TPP.
Practical Significance:
At present, TPP has been applied to identify the targets and off-targets of drugs and interrogate protein-metabolite and protein-protein interactions.
📋 中文结构化总结 Chinese Structured Summary
背景:
热蛋白质组分析（TPP）是细胞热转移实验（CETSA）与定量质谱（MS）的结合，也称为MS-CETSA。TPP通过测量细胞或细胞裂解液在不同加热温度下可溶性蛋白的含量来确定整个蛋白质组的稳定性。当蛋白质与小分子（如药物或代谢物）、核酸或其他蛋白质相互作用或发生翻译后修饰时，其热稳定性会发生变化，而TPP可以根据有无配体结合时热稳定性的差异来鉴定靶蛋白。
方法:
不适用——综述文章
结果:
TPP可以根据有无配体结合时热稳定性的差异来鉴定靶蛋白。目前，TPP已被应用于鉴定药物的靶点及脱靶蛋白，并用于研究蛋白质-代谢物及蛋白质-蛋白质相互作用。
数据概要:
TPP通过测量细胞或细胞裂解液在不同加热温度下可溶性蛋白的含量来确定整个蛋白质组的稳定性。
结论:
由于目前对该技术的认识有限，本综述介绍了TPP的原理、方法、应用、优势及局限性。
实际意义:
目前，TPP已被应用于鉴定药物的靶点及脱靶蛋白，并用于研究蛋白质-代谢物及蛋白质-蛋白质相互作用。