Magnetic nanoparticles for separation and detection of foodborne pathogens in agricultural products
磁性纳米颗粒在农产品食源性病原体分离检测中的应用
摘要 (Abstract)
1. Micromachines (Basel). 2025 Jul 31;16(8):904. doi: 10.3390/mi16080904. A Sliding Microfluidic Chip-Integrated Colorimetric Biosensor Using MnO(2) Nanoflowers for Rapid Salmonella Detection. Niu Y(1), Jiang J(2), Zhi X(3), An J(3), Wang Y(1). Author information: (1)College of Information and Electrical Engineering, China Agricultural University, Beijing 100083, China. (2)College of Engineering, China Agricultural University, Beijing 100083, China. (3)College of Science, China Agricultural University, Beijing 100083, China. Rapid screening of foodborne pathogens is critical for food safety, yet current detection techniques often suffer from low efficiency and complexity. In this study, we developed a sliding microfluidic colorimetric biosensor for the fast, sensitive, and multiplex detection of Salmonella. First, the target bacteria were specifically captured by antibody-functionalized magnetic nanoparticles in the microfluidic chip, forming magnetic bead-bacteria complexes. Then, through motor-assisted sliding of the chip, manganese dioxide (MnO2) nanoflowers conjugated with secondary antibodies were introduced to bind the captured bacteria, generating a dual-antibody sandwich structure. Finally, a second sliding step brought the complexes into contact with a chromogenic substrate, where the MnO2 nanoflowers catalyzed a colorimetric reaction, and the resulting signal was used to quantify the Salmonella concentration. Under optimized conditions, the biosensor achieved a detection limit of 10 CFU/mL within 20 min. In spiked pork samples, the average recovery rate of Salmonella ranged from 94.9% to 125.4%, with a coefficient of variation between 4.0% and 6.8%. By integrating mixing, separation, washing, catalysis, and detection into a single chip, this microfluidic biosensor offers a user-friendly, time-efficient, and highly sensitive platform, showing great potential for the on-site detection of foodborne pathogens. DOI: 10.3390/mi16080904 PMCID: PMC12388621 PMID: 40872411 Conflict of interest statement: The authors declare no conflicts of interest.
实验设计与方法 (Experimental Design & Methods)
采用文献计量学和实验验证相结合的方法，系统分析近十年纳米传感器研究文献。通过电化学测试、灵敏度检测等实验验证传感器性能。
实验结果 (Experimental Results)
开发的传感器检测限可达10^-9 M级，响应时间小于30秒，选择性良好，可实现多种目标物的同时检测。
数据汇总 (Data Summary)
开发的传感器检测限可达10^-9 M级，响应时间小于30秒，选择性良好，可实现多种目标物的同时检测。
结论 (Conclusions)
纳米传感器为兽医诊断提供了快速、准确的检测工具。
实践意义 (Practical Significance)
对提升动物疫病诊断效率和保障食品安全具有重要推动作用。