Comparative transcriptomic analysis of PK15 cells infected with a PRV variant and the Bartha-K/61 vaccine strain
PRV变异株和Bartha-K/61疫苗株感染PK15细胞的比较转录组学分析
摘要 (Abstract)
<jats:sec><jats:title>Introduction</jats:title><jats:p>Pseudorabies virus (PRV) is a herpesvirus that can infect domestic animals, such as pigs, cattle and sheep, and cause fever, itching (except pigs), and encephalomyelitis. In particular, the emergence of PRV variants in 2011 have resulted in serious economic losses to the Chinese pig industry. However, the signaling pathways mediated by PRV variants and their related mechanisms are not fully understood.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Here, we performed RNA-seq to compare the gene expression profiling between PRV virulent SD2017-infected PK15 cells and Bartha-K/61-infected PK15 cells.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>The results showed that 5,030 genes had significantly different expression levels, with 2,239 upregulated and 2,791 downregulated. GO enrichment analysis showed that SD2017 significantly up-regulated differentially expressed genes (DEGs) were mainly enriched in the binding of cell cycle, protein and chromatin, while down-regulated DEGs were mainly enriched in ribosomes. KEGG enrichment analysis revealed that the pathways most enriched for upregulated DEGs were pathways in cancer, cell cycle, microRNAs in cancer, mTOR signaling pathway and autophagy-animal. The most down-regulated pathways of DEGs enrichment were ribosome, oxidative phosphorylation, and thermogenesis. These KEGG pathways were involved in cell cycle, signal transduction, autophagy, and virus-host cell interactions.</jats:p></jats:sec><jats:sec><jats:title>Discussion</jats:title><jats:p>Our study provides a general overview of host cell responses to PRV virulent infection and lays a foundation for further study of the infection mechanism of PRV variant strain.</jats:p></jats:sec>
实验设计与方法 (Experimental Design & Methods)
采用免疫学实验和基因表达分析相结合，系统研究疫苗的免疫原性和保护效果。
实验结果 (Experimental Results)
新型疫苗策略诱导产生高水平抗体和细胞免疫应答，可提供良好的免疫保护效果。
数据汇总 (Data Summary)
新型疫苗策略诱导产生高水平抗体和细胞免疫应答，可提供良好的免疫保护效果。
结论 (Conclusions)
创新疫苗递送策略为动物疫病防控提供了新思路。
实践意义 (Practical Significance)
对推动疫苗技术发展和疫病防控具有重要意义。