Advancing coenzyme Q10 delivery with plant protein-based nanoparticle-mediated nanosuspensions.
利用植物蛋白纳米颗粒介导的纳米混悬剂推进辅酶Q10递送
📄 英文摘要 English Abstract
Coenzyme Q10 (CoQ10) possesses significant health-promoting potential, yet its oral delivery encounters obstacles stemming from its distinctive physicochemical characteristics, such as poor solubility, sensitivity to environmental factors and low bioaccessibility. To overcome these challenges, we developed high-payload CoQ10 nanosuspensions (CQ@SPNP, CQ@RPNP, and CQ@WPNP) using plant-based protein nanoparticles (NPs) derived from soybean (SPNP), rice (RPNP), and walnut (WPNP). The nanosuspensions include spherical particles, characterized by small particle size (<230 nm), low polydispersity (PDI < 0.15), and a high zeta potential (<-44 mV). CoQ10 loading capacity exceeded 70.3 %, with an encapsulation efficiency of over 77.4 %. CoQ10 interacted with plant protein-based NPs via hydrophobic effect without losing its crystal structure. Moreover, SPNP, RPNP, and WPNP significantly increased the stability of CoQ10 nanosuspensions against light, heat, long-term storage, and in vitro digestion. In particular, CQ@WPNP exhibited the highest stability and CoQ10 bioaccessibility post-digestion. The observed increases in stability and bioaccessibility were closely related to the specific NPs utilized. This study highlights the potential of plant protein-based NPs in addressing challenges of CoQ10 delivery, offering a promising approach to improve its efficacy.
📄 中文摘要 Chinese Abstract
📋 英文结构化总结 English Structured Summary
摘要整理
Background:
Coenzyme Q10 (CoQ10) possesses significant health-promoting potential, yet its oral delivery encounters obstacles stemming from its distinctive physicochemical characteristics, such as poor solubility, sensitivity to environmental factors and low bioaccessibility.
Methods:
To overcome these challenges, we developed high-payload CoQ10 nanosuspensions (CQ@SPNP, CQ@RPNP, and CQ@WPNP) using plant-based protein nanoparticles (NPs) derived from soybean (SPNP), rice (RPNP), and walnut (WPNP).
Results:
The nanosuspensions include spherical particles, characterized by small particle size (<230 nm), low polydispersity (PDI < 0.15), and a high zeta potential (<-44 mV). CoQ10 interacted with plant protein-based NPs via hydrophobic effect without losing its crystal structure. Moreover, SPNP, RPNP, and WPNP significantly increased the stability of CoQ10 nanosuspensions against light, heat, long-term storage, and in vitro digestion. In particular, CQ@WPNP exhibited the highest stability and CoQ10 bioaccessibility post-digestion. The observed increases in stability and bioaccessibility were closely related to the specific NPs utilized.
Data Summary:
CoQ10 loading capacity exceeded 70.3 %, with an encapsulation efficiency of over 77.4 %. Particle size <230 nm, PDI < 0.15, zeta potential <-44 mV.
Conclusions:
This study highlights the potential of plant protein-based NPs in addressing challenges of CoQ10 delivery, offering a promising approach to improve its efficacy.
Practical Significance:
This study highlights the potential of plant protein-based NPs in addressing challenges of CoQ10 delivery, offering a promising approach to improve its efficacy.
📋 中文结构化总结 Chinese Structured Summary
背景:
辅酶Q10（CoQ10）具有显著的健康促进潜力，但其口服递送面临由其独特理化特性引发的障碍，例如溶解度差、对环境因素敏感以及生物可及性低。
方法:
为克服这些挑战，我们利用来源于大豆（SPNP）、大米（RPNP）和核桃（WPNP）的植物蛋白纳米颗粒（NPs）开发了高载药量的CoQ10纳米混悬液（CQ@SPNP、CQ@RPNP和CQ@WPNP）。
结果:
纳米混悬液包含球形颗粒，其特征为粒径小（<230 nm）、低多分散性（PDI < 0.15）和高Zeta电位（<-44 mV）。CoQ10通过疏水作用与植物蛋白基纳米颗粒相互作用，且未丧失其晶体结构。此外，SPNP、RPNP和WPNP显著提高了CoQ10纳米混悬液在光照、热、长期储存及体外消化过程中的稳定性。其中，CQ@WPNP在消化后表现出最高的稳定性和CoQ10生物可及性。所观察到的稳定性和生物可及性的提升与所使用的特定纳米颗粒密切相关。
数据摘要:
CoQ10载药量超过70.3%，包封率高于77.4%。粒径<230 nm，PDI<0.15，Zeta电位<-44 mV。
结论:
本研究强调了植物蛋白基纳米颗粒在解决CoQ10递送挑战方面的潜力，为提高其功效提供了一种有前景的方法。
实际意义:
本研究强调了植物蛋白基纳米颗粒在解决CoQ10递送挑战方面的潜力，为提高其功效提供了一种有前景的方法。