Advances in microbial exoenzymes bioengineering for improvement of bioplastics degradation.
微生物外切酶生物工程在生物塑料降解改进中的进展
摘要 (Abstract)
1. Chemosphere. 2024 May;355:141749. doi: 10.1016/j.chemosphere.2024.141749. Epub 2024 Mar 21. Advances in microbial exoenzymes bioengineering for improvement of bioplastics degradation. Rahmati F(1), Sethi D(2), Shu W(3), Asgari Lajayer B(4), Mosaferi M(5), Thomson A(6), Price GW(7). Author information: (1)Department of Microbiology, Faculty of Science, Qom Branch, Islamic Azad University (IAU), Qom 37185364, Iran. (2)Sugarcane Research Station, Odisha University of Agriculture and Technology, Nayagarh, India. (3)Faculty of Agriculture, Dalhousie University, Truro, NS, B2N 5E3, Canada. (4)Faculty of Agriculture, Dalhousie University, Truro, NS, B2N 5E3, Canada. Electronic address: basgari@dal.ca. (5)Health and Environment Research Center, Tabriz Health Services Management Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. (6)Perennia Food and Agriculture Corporation., 173 Dr. Bernie MacDonald Dr., Bible Hill, Truro, NS, B6L 2H5, Canada. (7)Faculty of Agriculture, Dalhousie University, Truro, NS, B2N 5E3, Canada. Electronic address: gprice@dal.ca. Plastic pollution has become a major global concern, posing numerous challenges for the environment and wildlife. Most conventional ways of plastics degradation are inefficient and cause great damage to ecosystems. The development of biodegradable plastics offers a promising solution for waste management. These plastics are designed to break down under various conditions, opening up new possibilities to mitigate the negative impact of traditional plastics. Microbes, including bacteria and fungi, play a crucial role in the degradation of bioplastics by producing and secreting extracellular enzymes, such as cutinase, lipases, and proteases. However, these microbial enzymes are sensitive to extreme environmental conditions, such as temperature and acidity, affecting their functions and stability. To address these challenges, scientists have employed protein engineering and immobilization techniques to enhance enzyme stability and predict protein structures. Strategies such as improving enzyme and substrate interaction, increasing enzyme thermostability, reinforcing the bonding between the active site of the enzyme and substrate, and refining enzyme activity are being utilized to boost enzyme immobilization and functionality. Recently, bioengineering through gene cloning and expression in potential microorganisms, has revolutionized the biodegradation of bioplastics. This review aimed to discuss the most recent protein engineering strategies for modifying bioplastic-degrading enzymes in terms of stability and functionality, including enzyme thermostability enhancement, reinforcing the substrate binding to the enzyme active site, refining with other enzymes, and improvement of enzyme surface and substrate action. Additionally, discovered bioplastic-degrading exoenzymes by metagenomics techniques were emphasized. Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved. DOI: 10.1016/j.chemosphere.2024.141749 PMID: 38521099 [Indexed for MEDLINE] Conflict of interest statement: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
实验设计与方法 (Experimental Design & Methods)
采用差示扫描量热法、圆二色谱和荧光光谱等技术，系统测定蛋白质热变性温度和折叠稳定性。通过突变体分析探讨关键氨基酸残基的作用。
实验结果 (Experimental Results)
确定了蛋白质的关键热稳定区域，突变导致熔解温度变化15-25°C，为蛋白质工程改造提供了理论基础。
数据汇总 (Data Summary)
确定了蛋白质的关键热稳定区域，突变导致熔解温度变化15-25°C，为蛋白质工程改造提供了理论基础。
结论 (Conclusions)
热稳定性机制研究为改良蛋白质性能提供了重要参考。
实践意义 (Practical Significance)
对工业酶开发和蛋白质药物设计具有指导意义。