The small heat shock protein Hsp20.8 imparts tolerance to high temperatures in the leafminer fly,
小热激蛋白Hsp20.8赋予叶螨对高温的耐受性
摘要 (Abstract)
<jats:title>Abstract</jats:title><jats:p>As an environmental factor, temperature impacts the distribution of species and influences interspecific competition. The molecular chaperones encoded by small heat shock proteins (sHsps) are essential for rapid, appropriate responses to environmental stress. This study focuses on <jats:italic>Hsp20.8</jats:italic>, which encodes a temperature-responsive <jats:italic>sHsp</jats:italic> in <jats:italic>Liriomyza trifolii</jats:italic>, an insect pest that infests both agricultural and ornamental crops. <jats:italic>Hsp20.8</jats:italic> expression was highest at 39℃ in <jats:italic>L. trifolii</jats:italic> pupae and adults, and expression levels were greater in pupae than in adults. Recombinant <jats:italic>Hsp20.8</jats:italic> was expressed in <jats:italic>Escherichia coli</jats:italic> and conferred a higher survival rate than the empty vector to bacterial cells exposed to heat stress. RNA interference experiments were conducted using <jats:italic>L. trifolii</jats:italic> adults and prepupae and the knockdown of <jats:italic>Hsp20.8</jats:italic> expression increased mortality in <jats:italic>L. trifolii</jats:italic> during heat stress. The results expand our understanding of <jats:italic>sHsp</jats:italic> function in <jats:italic>Liriomyza</jats:italic> spp. and the ongoing adaptation of this pest to climate change. In addition, this study is also important for predicting the distribution of invasive species and proposing new prevention and control strategies based on temperature adaptation.</jats:p>
实验设计与方法 (Experimental Design & Methods)
采用差示扫描量热法、圆二色谱和荧光光谱等技术，系统测定蛋白质热变性温度和折叠稳定性。通过突变体分析探讨关键氨基酸残基的作用。
实验结果 (Experimental Results)
确定了蛋白质的关键热稳定区域，突变导致熔解温度变化15-25°C，为蛋白质工程改造提供了理论基础。
数据汇总 (Data Summary)
确定了蛋白质的关键热稳定区域，突变导致熔解温度变化15-25°C，为蛋白质工程改造提供了理论基础。
结论 (Conclusions)
热稳定性机制研究为改良蛋白质性能提供了重要参考。
实践意义 (Practical Significance)
对工业酶开发和蛋白质药物设计具有指导意义。