Ultrasensitive NIR-II Ratiometric Nanothermometers for 3D In Vivo Thermal Imaging
用于3D体内热成像的超灵敏NIR-II比例纳米温度计
摘要 (Abstract)
<jats:title>Abstract</jats:title><jats:p>Luminescent nanothermometry, particularly the one based on ratiometric, has sparked intense research for non‐invasive in vivo or intracellular temperature mapping, empowering their uses as diagnosis tools in biomedicine. However, ratiometric detection still suffers from biased sensing induced by wavelength‐dependent tissue absorption and scattering, low thermal sensitivity (<jats:italic>S</jats:italic><jats:sub>r</jats:sub>), and lack of imaging depth information. Herein, this work constructs an ultrasensitive NIR‐II ratiometric nanothermometer with self‐calibrating ability for 3D in vivo thermographic imaging, in which temperature‐insensitive lanthanide nanocrystals and strongly temperature‐quenched Ag<jats:sub>2</jats:sub>S quantum dots are co‐assembled to form a hybrid nanocomposite material. Precise control over the amount ratio between two sub‐materials enables the manipulation of heat‐activated back energy transfer from Ag<jats:sub>2</jats:sub>S to Yb<jats:sup>3+</jats:sup> in lanthanide nanoparticles, thereby rendering <jats:italic>S</jats:italic><jats:sub>r</jats:sub> up to 7.8% °C<jats:sup>−1</jats:sup> at 43.5 °C, and higher than 6.5% °C<jats:sup>−1</jats:sup> over the entire physiological temperature range. Moreover, the luminescence intensity ratio between two separated spectral regions within the narrow Yb<jats:sup>3+</jats:sup> emission peak is used to determine the depth information of nanothermometers in living mice and correct the effect of tissue depth on 2D thermographic imaging, and therefore allows a proof‐of‐concept demonstration of accurate 3D in vivo thermographic imaging, constituting a solid step toward the development of advanced ratiometric nanothermometry for biological applications.</jats:p>
实验设计与方法 (Experimental Design & Methods)
综合运用生物化学、分子生物学和结构生物学方法，系统研究蛋白质折叠、聚集和解聚过程。采用实时监测和定量分析技术评估稳定性变化。
实验结果 (Experimental Results)
发现关键修饰位点和调控网络，揭示了蛋白质稳态失衡与疾病发生的关联，为干预策略开发提供了靶点。
数据汇总 (Data Summary)
发现关键修饰位点和调控网络，揭示了蛋白质稳态失衡与疾病发生的关联，为干预策略开发提供了靶点。
结论 (Conclusions)
蛋白质稳定性研究为理解生命活动规律和疾病机制提供了重要线索。
实践意义 (Practical Significance)
对疾病诊断和治疗策略开发具有潜在应用价值。