Spray Drying of Bacterial Membrane Vesicles for Vaccine Delivery.
细菌膜囊泡的喷雾干燥用于疫苗递送
📄 英文摘要 English Abstract
Extracellular vesicles are nanosized lipid-bilayered spheres secreted from every living cell and they serve physiological and pathophysiological functions. Bacterial membrane vesicles are shed from both Gram-negative and Gram-positive bacteria and harbor many virulence factors, nuclear material, polysaccharides, proteins, and antigenic determinants, which are essential for immune recognition and evasion. Hence, bacterial membrane vesicles are very promising vaccine candidates. Spray drying is a well-established pharmaceutical technique to produce inhalable dry powders with enhanced stability for formulations of vaccines. In this chapter, we illustrate general guidelines for spray drying of bacterial extracellular vesicles to improve their stability without compromising their immunogenic protective effect. We discuss some of the most important experiments to characterize the generated spray-dried bacterial membrane vesicle powder vaccine.
📄 中文摘要 Chinese Abstract
📋 英文结构化总结 English Structured Summary
摘要整理
1.
Background:
Extracellular vesicles are nanosized lipid-bilayered spheres secreted from every living cell and they serve physiological and pathophysiological functions. Bacterial membrane vesicles are shed from both Gram-negative and Gram-positive bacteria and harbor many virulence factors, nuclear material, polysaccharides, proteins, and antigenic determinants, which are essential for immune recognition and evasion. Hence, bacterial membrane vesicles are very promising vaccine candidates.
2.
Methods:
Spray drying is a well-established pharmaceutical technique to produce inhalable dry powders with enhanced stability for formulations of vaccines. In this chapter, we illustrate general guidelines for spray drying of bacterial extracellular vesicles to improve their stability without compromising their immunogenic protective effect. We discuss some of the most important experiments to characterize the generated spray-dried bacterial membrane vesicle powder vaccine.
3.
Results:
We discuss some of the most important experiments to characterize the generated spray-dried bacterial membrane vesicle powder vaccine.
4.
Data Summary:
No quantitative data are provided in the text.
5.
Conclusions:
The text does not provide explicit conclusions; the stated aim is to improve stability without compromising the immunogenic protective effect.
6.
Practical Significance:
Bacterial membrane vesicles are very promising vaccine candidates. Spray drying is a well-established pharmaceutical technique to produce inhalable dry powders with enhanced stability for formulations of vaccines.
📋 中文结构化总结 Chinese Structured Summary
背景:
细胞外囊泡是由活细胞分泌的纳米级脂质双层球体，具有生理和病理生理功能。细菌膜囊泡由革兰氏阴性菌和革兰氏阳性菌释放，携带多种毒力因子、核酸物质、多糖、蛋白质和抗原决定簇，这些成分对免疫识别和免疫逃逸至关重要。因此，细菌膜囊泡是非常有前景的疫苗候选物。
方法:
喷雾干燥是一种成熟的制药技术，可用于生产具有增强稳定性的可吸入干粉疫苗制剂。在本章中，我们阐述了喷雾干燥细菌细胞外囊泡的一般指南，以提高其稳定性而不损害其免疫保护效果。我们讨论了表征所制备的喷雾干燥细菌膜囊粉疫苗的一些最重要实验。
结果:
我们讨论了表征所制备的喷雾干燥细菌膜囊粉疫苗的一些最重要实验。
数据摘要:
文中未提供定量数据。
结论:
文中未给出明确结论；所述目标是在不损害免疫保护效果的前提下提高稳定性。
实际意义:
细菌膜囊是非常有前景的疫苗候选物。喷雾干燥是一种成熟的制药技术，可用于生产具有增强稳定性的可吸入干粉疫苗制剂。