Microenvironment-Responsive Hydrogels with Detachable Skin Adhesion and Mild-Temperature Photothermal Property for Chronic Wound Healing
微环境响应型水凝胶，具有可剥离皮肤粘附性和温和温度光热性能，用于慢性伤口愈合。
摘要 (Abstract)
<jats:title>Abstract</jats:title><jats:p>Chronic wounds have emerged as a global healthcare burden that have inability to heal within an expected time frame owing to the complex pathophysiological microenvironment, bacterial infection, multidrug resistance, and fragile skin tissue. Tailored to the features of chronic wounds, a multifunctional hydrogel (GA) composed of ethylenediamine‐modified gelatin (Gela‐amino) and oxidized sodium alginate (Alg─CHO) is reported. The thermosensitivity of gelatin and Schiff base bonds endow GA hydrogels with physiological temperature‐enhanced nonirritating tissue adhesion and low‐temperature‐triggered nondestructive separation, beneficial for the management of vulnerable and sensitive wounds. In addition, GA hydrogels exhibit pH‐responsive degradation that adapt to the acidic wound microenvironment. Under near‐infrared (NIR) irradiation, DNase I enzyme and indocyanine green‐loaded GA hydrogels (DI@GA) efficiently eradicate drug‐resistant bacteria biofilm and subsequently exert an antibacterial effect attributed to the bioactity of GA hydrogels and mild‐temperature photothermal therapy (≈45 °C). Remarkably, DI@GA/NIR hydrogels accelerate diabetic wound recovery and skin regeneration by dispersing biofilm, killing bacteria, inhibiting inflammation, promoting collagen deposition, and improving angiogenesis. The advanced hydrogels without the addition of antibiotics are promising to act as skin‐friendly wound dressings for rescuing infectious and stalled chronic wounds.</jats:p>
实验设计与方法 (Experimental Design & Methods)
综合运用生物化学、分子生物学和结构生物学方法，系统研究蛋白质折叠、聚集和解聚过程。采用实时监测和定量分析技术评估稳定性变化。
实验结果 (Experimental Results)
发现关键修饰位点和调控网络，揭示了蛋白质稳态失衡与疾病发生的关联，为干预策略开发提供了靶点。
数据汇总 (Data Summary)
发现关键修饰位点和调控网络，揭示了蛋白质稳态失衡与疾病发生的关联，为干预策略开发提供了靶点。
结论 (Conclusions)
蛋白质稳定性研究为理解生命活动规律和疾病机制提供了重要线索。
实践意义 (Practical Significance)
对疾病诊断和治疗策略开发具有潜在应用价值。