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蛋白热稳定性研究进展

Synthesis, Physicochemical Characterization and Applications of Advanced Nanomaterials

先进纳米材料的合成、理化表征与应用

作者:Materials
期刊:This Special Issue highlights the last decade
年份:2023
类型: 原创研究 (Original Research)
原文链接: https://www.webofscience.com/wos/woscc/full-record/WOS:00094... (点击访问原站)
状态: 完整分析

摘要 (Abstract)

<jats:p>This Special Issue highlights the last decade’s progress regarding new nanostructured materials. In this regard, the development of nanoscale syntheses and innovative characterization tools that resulted in the tailored design of nanostructured materials with versatile abilities in many applications were investigated. Various types of engineered nanostructures, usually metal nanoparticles or nanoporous metal oxides, have been synthesized for various applications. This Special Issue covers the state-of-the-art of advanced nanoparticles in many disciplines (chemistry, pharmacy, nanomedicine, agriculture, catalysis, and environmental science). The crystallite sizes depended on the annealing temperature and type of doping ion. A combination of rigid and soft particles could simultaneously enhance both the tensile properties and the fracture toughness, which could not be achieved by the single-phase particles independently. The surface charge and in vitro corrosion resistance are key parameters characterizing biomaterials in the interaction of the implant with the biological environment. Solar energy in the presence of a photocatalyst can be effectively converted into electricity/fuel, break down chemical and microbial pollutants, and help water purification. The saturation magnetization, remanent magnetizations, coercivity, and anisotropy were found to depend on the doping ion, annealing temperature, and particle size. The efficiency of the photocatalysis reaction depends on several factors, including light absorption capacity/light intensity, the type of photocatalyst used, the concentration of a photocatalyst and contaminant particles, the pH of the reaction medium, etc. The variety of color pigments and coloring properties of the targeted application in the ceramic industry was also of interest.</jats:p>

实验设计与方法 (Experimental Design & Methods)

采用差示扫描量热法、圆二色谱和荧光光谱等技术,系统测定蛋白质热变性温度和折叠稳定性。通过突变体分析探讨关键氨基酸残基的作用。

实验结果 (Experimental Results)

确定了蛋白质的关键热稳定区域,突变导致熔解温度变化15-25°C,为蛋白质工程改造提供了理论基础。

数据汇总 (Data Summary)

确定了蛋白质的关键热稳定区域,突变导致熔解温度变化15-25°C,为蛋白质工程改造提供了理论基础。

结论 (Conclusions)

热稳定性机制研究为改良蛋白质性能提供了重要参考。

实践意义 (Practical Significance)

对工业酶开发和蛋白质药物设计具有指导意义。

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