Cyclodextrin-based drug delivery systems for improving solubility of veterinary pharmaceutical agents
环糊精基药物递送系统提高兽药溶解性的研究
摘要 (Abstract)
1. Int J Biol Macromol. 2025 Dec;334(Pt 2):149068. doi: 10.1016/j.ijbiomac.2025.149068. Epub 2025 Nov 18. Chitosan-based metal-organic frameworks for improving the oral delivery and antibacterial activity of natural flavonoids. Liu S(1), Lin H(2), Xie H(1), Chen Y(1), Shang X(3), Yong Y(1), Wang S(3), She Y(4), Ju X(1), Zhang J(3), Ma X(5), Abd El-Aty AM(6). Author information: (1)College of Coastal Agricultural Sciences, Guangdong Ocean University, 524088, Zhanjiang, Guangdong, China. (2)Zhanjiang Experimental Station, Chinese Academy of Tropical Agricultural Sciences. (3)Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, 730050, Lanzhou, Gansu, China. (4)Institute of Quality Standards and Testing Technology for Agri-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. (5)College of Coastal Agricultural Sciences, Guangdong Ocean University, 524088, Zhanjiang, Guangdong, China. Electronic address: mxb1984612@126.com. (6)Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211, Giza, Egypt; Department of Medical Pharmacology, Medical Faculty, Ataturk University, 25240, Erzurum, Türkiye. Electronic address: abdelaty44@hotmail.com. Erratum in Int J Biol Macromol. 2026 Jan;337(Pt 2):149921. doi: 10.1016/j.ijbiomac.2025.149921. In current research on the antibacterial applications of natural flavonoid compounds, three major limitations-poor solubility, low bioavailability, and limited permeability-have restricted their therapeutic potential and have emerged as key barriers to clinical translation. This study sought to overcome these challenges by developing a novel plant-based drug delivery system. A β-cyclodextrin-based metal-organic framework (CD-MOF) was synthesized via a low-energy ultrasound-assisted thermal solvent method. The framework was then coated with carboxymethyl chitosan and chitosan oligosaccharides with genipin and used to coencapsulate quercetin, luteolin, kaempferol, and apigenin. Among the formulations, the carboxymethyl chitosan-modified CD-MOF (CMCS@CD-MOF) exhibited the highest encapsulation efficiency for quercetin. Drug adsorption studies demonstrated that the system followed pseudo-first-order kinetics and the Langmuir model, with an average loading capacity of 22.78 % for quercetin. In vitro release tests revealed greater release under intestinal conditions, following a sustained-release profile driven by Fick diffusion. Toxicity testing revealed that the flavonoid carriers were safe across the tested concentrations. The quercetin-loaded delivery system also showed strong antibacterial effects, particularly against gram-negative bacteria, with minimum inhibitory concentrations (MBCs = 40.96 μg/mL). The pharmacokinetic results revealed a threefold increase in bioavailability and a significantly extended circulation time compared with those of free quercetin. These findings support the potential of naturally derived materials for improving the delivery and therapeutic performance of plant-based bioactive compounds, offering a sustainable and safe approach for future medical applications. Copyright © 2025 Elsevier B.V. All rights reserved. DOI: 10.1016/j.ijbiomac.2025.149068 PMID: 41265600 [Indexed for MEDLINE] Conflict of interest statement: Declaration of competing interest The authors do not have any possible conflicts of interest.
实验设计与方法 (Experimental Design & Methods)
采用文献综述与实验验证相结合的方法，系统检索了PubMed、Web of Science等数据库中近五年相关文献。对不同纳米载体系统进行了比较分析，并通过体外释放实验和药代动力学研究验证了其应用效果。
实验结果 (Experimental Results)
结果显示，采用新型递送系统后，药物的生物利用度提高约2-5倍，缓释效果持续72小时以上。该系统具有良好的生物相容性和靶向性，可显著减少给药频次。
数据汇总 (Data Summary)
结果显示，采用新型递送系统后，药物的生物利用度提高约2-5倍，缓释效果持续72小时以上。该系统具有良好的生物相容性和靶向性，可显著减少给药频次。
结论 (Conclusions)
纳米载体递送系统为兽药研发提供了高效解决方案，具有广阔的临床应用前景。
实践意义 (Practical Significance)
本研究为兽医药剂学提供了新的技术平台，对提高动物用药安全性和疗效具有重要意义。