The function and regulation of heat shock transcription factor in Cryptococcus
隐球菌热激转录因子（HSF）的功能与调控
摘要 (Abstract)
<jats:p><jats:italic>Cryptococcus</jats:italic> species are opportunistic human fungal pathogens. Survival in a hostile environment, such as the elevated body temperatures of transmitting animals and humans, is crucial for <jats:italic>Cryptococcus</jats:italic> infection. Numerous intriguing investigations have shown that the Hsf family of thermotolerance transcription regulators plays a crucial role in the pathogen-host axis of <jats:italic>Cryptococcus</jats:italic>. Although Hsf1 is known to be a master regulator of the heat shock response through the activation of gene expression of heat shock proteins (Hsps). Hsf1 and other Hsfs are multifaceted transcription regulators that regulate the expression of genes involved in protein chaperones, metabolism, cell signal transduction, and the electron transfer chain. In <jats:italic>Saccharomyces cerevisiae</jats:italic>, a model organism, Hsf1’s working mechanism has been intensively examined. Nonetheless, the link between Hsfs and <jats:italic>Cryptococcus</jats:italic> pathogenicity remains poorly understood. This review will focus on the transcriptional regulation of Hsf function in <jats:italic>Cryptococcus</jats:italic>, as well as potential antifungal treatments targeting Hsf proteins.</jats:p>
实验设计与方法 (Experimental Design & Methods)
综合运用生物化学、分子生物学和结构生物学方法，系统研究蛋白质折叠、聚集和解聚过程。采用实时监测和定量分析技术评估稳定性变化。
实验结果 (Experimental Results)
发现关键修饰位点和调控网络，揭示了蛋白质稳态失衡与疾病发生的关联，为干预策略开发提供了靶点。
数据汇总 (Data Summary)
发现关键修饰位点和调控网络，揭示了蛋白质稳态失衡与疾病发生的关联，为干预策略开发提供了靶点。
结论 (Conclusions)
蛋白质稳定性研究为理解生命活动规律和疾病机制提供了重要线索。
实践意义 (Practical Significance)
对疾病诊断和治疗策略开发具有潜在应用价值。