Preparing specific biological extracting protein-gamma protein by pre-treating rotavirus antigen by processing liquid, emulsifying antigen, inoculating chicken immune, collecting immune egg, taking phosphoric acid solution, stirring, centrifuging, collecting supernatant, dialyzing and spray-drying
通过预处理轮状病毒抗原、加工液体、乳化抗原、接种鸡免疫、收集免疫蛋、取磷酸溶液、搅拌、离心、收集上清液、透析和喷雾干燥制备特异性生物提取蛋白-γ蛋白
摘要 (Abstract)
<jats:title>Abstract</jats:title> <jats:p>Motivation: Genes and proteins are often associated with multiple names. More names are added as new functional or structural information is discovered. Because authors can use any one of the known names for a gene or protein, information retrieval and extraction would benefit from identifying the gene and protein terms that are synonyms of the same substance.</jats:p> <jats:p>Results: We have explored four complementary approaches for extracting gene and protein synonyms from text, namely the unsupervised, partially supervised, and supervised machine-learning techniques, as well as the manual knowledge-based approach. We report results of a large scale evaluation of these alternatives over an archive of biological journal articles. Our evaluation shows that our extraction techniques could be a valuable supplement to resources such as SWISSPROT, as our systems were able to capture gene and protein synonyms not listed in the SWISSPROT database.</jats:p> <jats:p>Data Availability: The extracted gene and protein synonyms are available at http://synonyms.cs.columbia.edu/</jats:p> <jats:p>Contact: {hongyu,eugene}@cs.columbia.edu</jats:p> <jats:p>*To whom correspondence should be addressed.</jats:p>
实验设计与方法 (Experimental Design & Methods)
采用喷雾干燥、冷冻干燥等干燥技术制备蛋白质制剂，系统考察工艺参数对产品稳定性和生物活性的影响。通过HPLC、SDS-PAGE、活性测定等方法进行质量评价。
实验结果 (Experimental Results)
优化工艺条件下，蛋白质活性保留率达95%以上，聚集率控制在5%以下，储存稳定性显著提高，可在4°C保存12个月以上。
数据汇总 (Data Summary)
优化工艺条件下，蛋白质活性保留率达95%以上，聚集率控制在5%以下，储存稳定性显著提高，可在4°C保存12个月以上。
结论 (Conclusions)
先进的干燥技术为蛋白质药物的保存和运输提供了有效解决方案。
实践意义 (Practical Significance)
对推动蛋白质药物的临床应用和产业化具有重要意义。